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Publication datasheet
Title:
Next - generation sequencing: historical perspective and current applications in plant virology
Authors:
Hadidi, A.; Barba, M.
Year:
2012
Languages:
ENG, eng
Journal:
Petria
Kind of publication:
Elettronico
Location:
Roma
Editor:
CRA-PAV
Abstract in Italian:
Abstract in English:
Next-generation sequencing tecjnologies, also known as deep or high throughput sequencing, provide a very efficient and fast as well as low cost DNA sequencing beyond the reach of the standard and traditional DNA sequencing technologies developed in the late 1970ís. Historical events in the last few centuries that led to its development at the beginning of the 21 century are presented. Nextgeneration sequencing technologies have been recently applied to the field of plant virology. Application examples are presented, particularly in the areas of virus genome sequencing, ecology, discovery, epidemiology, transcriptomics, replication, detection and identification. Possible prospective for next-generation sequencing in revealing the etiology of the disease of unknown etiology in temperate fruit crops arealso discussed. Here we describe the milestones which have marked the evolution of the scientific studies in plant virology starting from the introduction of tobacco plants in Europe

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