Reverse transcription loop-mediated isothermal amplification (RT-LAMP): A novel method for the detection of Peach latent mosaic viroid (PLMVd)
Boubourakas, I.N., Fukuta, S.; Faggioli, F.; Luigi, M.; Barba, M.; Kyriakopoulou, P.E.
Proceedings 21th ICVF, International Conference on Viruses of Fruit Crops 2009, Neustadt am der Weinstrasse, Germany, 5-10 Luglio 2009.
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Peach latent mosaic viroid (PLMVd) is the causal agent of the economically important peach latent mosaic disease. In Greece, PLMVd has been reported for the first time in pear and wild pear, in 2001 and in 2004 in apricot, but there is no information on the presence of this viroid in its main host, peach and its germplasm. In this study the presence of PLMVd in peach and peach germplasm (nursery stock) was examined thoroughly, as well as in other Prunus species, and in pome fruit species. Leaf samples were collected from orchards in Pella and Imathia prefectures of Macedonia, and in Magnesia and Larissa and of Thessaly in Argolida and Piraeus prefectures of Peloponnesus and Attica, respectively. The presence of the viroid was ascertained by RT-PCR assays, slot-blot hybridization, nucleotide sequencing and RT-LAMP assays. RT-LAMP assays were used for fist time internationally for the detection of PLMVd. Peach (59/75), plum (9/24) apricot (5/15) and cherry (3/23) tree samples were found infected with PLMVd. It was interesting to see that more than 46% (107/231) of peach germplasm examined (nursery stock) was found infected. The viroid was also detected in pear, wild pear and quince samples.