Optimization and improvement of oligonucleotide microarray-based detection of tomato viruses and pospiviroids
Tiberini, A.; Barba, M.
Journal of Virological Methods
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Tomato (Solanum lycopersicum L.) is a vegetable crop which is affected by many viruses and several
viroids, causing significant economic loss. Their detection and identification is of critical importance for
plant protection and quarantine and certification programs.
The potential was examined of an array based on the Combimatrix platform for the detection of 37
viruses belonging to 13 families, one of which is unassigned, together with six pospiviroid species, genus
Pospiviroid, family Pospiviroidae.
More than 470 oligonucleotide probes (40-mer) were selected for the microarray diagnostic technique
developed in this investigation. Most of the virus probes were highly specific and were able to identify
tomato viruses. Most pospiviroid probes, however, were non-specific in terms of species, but were specific
at the genus level as they hybridized to members of the genus Pospiviroid. Only one probe of the Tomato
apical stem viroid was species specific.
The repeatability and specificity of the Combimatrix method showed that it can be considered for
routine diagnostic use in suspected tomato germplasm since it detected 37 viruses and one pospiviroid
at the species level and 5–6 pospiviroids at the genus level. The estimated cost for testing of a single
tomato virus is similar to or less than the cost of using ELISA.
© 2012 Elsevier B.V. All rights reserved.