Rapid identification of Trialeurodes vaporariorum, Bemisia tabaci (MEAM1 and MED) and tomato-infecting criniviruses in whiteflies and in tomato leaves by real-time reverse transcription-PCR assay
Cavalieri, V.; Manglli, A.; Tiberini, A.; Tomassoli, L.; Rapisarda, C.
Bulletin of Insectology
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UniversitÓ di Bologna
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|Abstract in English:|
The whiteflies Bemisia tabaci and Trialeurodes vaporariorum (Hemiptera Aleyrodidae) are harmful pests of vegetable and ornamental
crops in many countries. Also, they are vectors of emergent viruses on tomato including the criniviruses (Closteroviridae
genus Crinivirus) Tomato chlorosis virus (ToCV) and Tomato infectious chlorosis virus (TICV). Since different vectors are involved
in the transmission of both viruses (ToCV is transmitted by B. tabaci, Trialeurodes abutiloneus and T. vaporariorum while
TICV is transmitted only by T. vaporariorum), and they induce similar symptoms on tomato plants, a sensitive and specific diagnosis
method is desirable. In addition, a rapid discriminating method of the vectors is essential for monitoring and control activities
and epidemiological studies. For these reasons, a combined protocol based on one-step multiplex real-time reverse transcription
(RT)-PCR has been developed for the identification of T. vaporariorum, two invasive species of the complex B. tabaci
(MEAM1 and MED) and for the specific detection of ToCV and TICV in whiteflies and plants.