|Riassunto in Inglese:|
Phytosanitary situation in ornamental plant industries is always very dynamic and changing due to globalization market, introduction of new plant genera, species or varieties and new cultural techniques (that can favour or repress different pathogens). Monitoring this kind of phytosanitary situation prove somehow difficult, so, with the ultimate aim of developing new diagnosis technologies based on molecular tools, molecular analysis of pathogens were performed in ornamental plant industries in Tuscany. We focused on woody plants, conifers (like Cupressus sp., Pinus sp or Taxus sp.) or not (Acer sp., Viburnum sp. or Pittosporum) affected by root rot and/or tracheomycoses collected in 2010. A total of 85 symptomatic samples were collected and analysed: methods were set up to extract DNA from woody material and perform efficient PCR with various primers on material of different plant origin. PCR with ITS5/ITS4 primers were performed to amplify fungi and with ITS6/ITS4 primers to amplify oomycetes. The first couple of primer amplify 33 samples out of 85, whereas the second one amplify 41 samples/85. Some samples have multiple bands (two or more) i.e. there was more than one fungus that could be pathogenic or just saprophytic. All the samples have also been amplified with primers specific for most common fungi, namely for Phytophthora sp., Cylindrocarpon sp. and also Phomopsis sp. Some samples were also sequenced to confirm PCR results or to unravel other fungi.