ITALIANO HIGH ACCESSIBILITY
Torna alla Home Page Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria Search in the site...  

European Charter for Researchers      HR EXCELLENCE IN RESEARCH
CREA - Via Po, 14 - 00198 ROMA
P.IVA: 08183101008 - C.F.: 97231970589
Tel: +39 06 478361 - Fax: +39 06 47836320 -
Posta Elettronica Certificata:

CIVIC ACCESS PRESS REVIEW URP JOB OPPORTUNITIES CONTRACTS TRANSPARENT ADMINISTRATION

freccina You are here: Home->Publications->Datasheet


Publication datasheet
Title:
Histological and molecular analysis of Rdg2a barley resistance to leaf stripe
Authors:
Haegi, A.; Bonardi, V.; Dall’Aglio, E.; Glissant, D.; Tumino, G.; Bulgarelli, D.; Collins, N.C.; Infantino, A.; Stanca, A.M.; Delledonne, M.; Valè, G.
Year:
2008
Languages:
ENG, eng
Journal:
Molecular Plant Pathology
Kind of publication:
Elettronico
Location:
Editor:
Wiley Blackwell
Abstract in Italian:
Abstract in English:
Barley (Hordeum vulgare L.) leaf stripe is caused by the seed borne fungus Pyrenophora graminea. We investigated microscopically and molecularly the reaction of barley embryos to leaf stripe inoculation. In the resistant genotype NIL3876-Rdg2a, fungal growth ceased at the scutellar node of the embryo, while in the susceptible near-isogenic line (NIL) Mirco-rdg2a fungal growth continued past the scutellar node and into the embryo. Pathogen-challenged embryos of resistant and susceptible NILs showed different levels of UV autofluorescence and toluidine blue staining, indicating differential accumulation of phenolic compounds. Suppression subtractive hybridisation (SSH) and cDNA-AFLP analyses of embryos identified P. graminea-induced and -repressed barley genes. In addition, cDNA-AFLP analysis identified six pathogenicity-associated fungal genes expressed during barley infection but at low to undetectable levels during growth on artificial media. Microarrays representing the entire set of differentially expressed cDNA-AFLP fragments and 100 barley homologues of previously described defence-related genes were used to study gene expression changes at 7 and 14 days after inoculation (dai) in the resistant and susceptible NILs. A total of 172 significantly modulated barley genes were identified and assigned to four groups based on timing and genotype dependence of expression. Analysis of the changes in gene expression during the barley resistance response to leaf stripe suggests that the Rdg2a-mediated response includes cell wall reinforcement, signal transduction, generation of ROS, cell protection, jasmonate signalling, and effector genes. The identification of genes showing leaf stripe inoculation or resistance dependent expression sets the stage for further dissection of the resistance response of barley embryo cells to leaf stripe.

AREA RISERVATA  Webmaster:
Logo mySQL Logo PHP