ENGLISH ACCESSIBILITA' ALTA
Torna alla Home Page Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria Cerca nel sito...  

Carta europea dei ricercatori      HR EXCELLENCE IN RESEARCH
CREA - Via Po, 14 - 00198 ROMA
P.IVA: 08183101008 - C.F.: 97231970589
Tel: +39 06 478361 - Fax: +39 06 47836320 -
Posta Elettronica Certificata:

ACCESSO CIVICO RASSEGNA STAMPA URP LAVORO/FORMAZIONE GARE/APPALTI AMMINISTRAZIONE TRASPARENTE

freccina Sei qui: Home->Pubblicazioni->Dettaglio POSTA


Scheda pubblicazione
Titolo:
Agrobacterium-mediated transformation of Pyrenochaeta lycopersici for investigating molecular determinants of virulence.
Autori:
Aragona, M.; Valente, M.T.; Infantino, A.
Anno:
2011
Lingue:
ENG, eng
Rivista:
Journal of Plant Pathology
Tipo di pubblicazione:
Cartaceo
Luogo:
Pisa
Editore:
Edizioni ETS
Riassunto in Italiano:
Riassunto in Inglese:
Pyrenochaeta lycopersici is a soil-borne fungus causing an economically important disease known as corky root rot on tomato and other hosts. A gene coding for an extracellular endo-1,4-bglucanase has recently been cloned and, in order to explore its putative role in virulence, an ATMT (Agrobacterium tumefaciensmediated transformation) system was established. P. lycopersici does not produce conidia in culture so the transformation method currently available for this species involves protoplast transformation in presence of PEG. Despite of the low efficiency, a GUS-expressing transformant has been obtained and used for investigating the timing of infection and colonization of P. lycopersici inside tomato roots. To increase the transformation efficiency and to obtain a larger number of transformants for gene replacement experiments, we tested both mycelium and protoplasts as recipients for an ATMT-based method. Preliminary results showed that only the protoplasts but not the mycelium were successfully transformed by using the pRF-HU2 vector, harbouring promoter and terminator genes from Aspergillus nidulans and hygromycin coding gene as fungal marker. The average transformation frequency obtained with the first experiments was up to 30 transformants per 107 protoplasts, all showing the DNA vector insertion. We are going to test the applicability of this technique for targeted gene replacement in P. lycopersici, after cloning the endo-1,4-b-glucanase sequences into the pRF-HU2 vector.

AREA RISERVATA  Webmaster:
Logo mySQL Logo PHP