|Riassunto in Inglese:|
The first published reports on detecting viruses by microarray technology appeared in 2002 and from that time the microarray-based approaches offer an alternative to the routine methods, i.e. ELISA, RT-PCR and/or qRT-PCR, as microarray virus-specific probes could be capable of detecting an almost unlimited number of virus species in one assay. Among these approaches, developing different microarray platforms, the microarray-based diagnosis methods, had been focused and aimed to identification of crop specific viruses or to develop an “universal” microarray to identify all the known viruses reported in plants.
The Combimatrix (Mukilteo, WA, USA) platform, recently, have showed all its potentiality to detect a large number of viruses in different plant matrices as tomato (Solanum lycopersicum L.) and globe artichoke (Cynara scolymus L.), allowing the identification of 37 viruses and 1 viroid and 14 viruses, respectively.
The repeatability and specificity of the Combimatrix platform showed that the developed method can be considered as a positive step for routine diagnostic use in suspected different germplasms, showing how the identification of plant viruses is limited only by the spectrum of the viruses and strain probes present in microarray. If from one hand, the increasing number of plant viruses nucleotide sequences may easily allow to design a great number of probes, from another hand, the possibility to test the quality these probes and their capability to identify its targets, requires a wide microorganism collection database.
In view of above, the upcoming and cutting edges technologies and diagnosis methods should go hand in hand with the creation and maintaining of microorganism collection databases.