ITALIANO HIGH ACCESSIBILITY
Torna alla Home Page Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria Search in the site...  

European Charter for Researchers      HR EXCELLENCE IN RESEARCH
CREA - Via Po, 14 - 00198 ROMA
P.IVA: 08183101008 - C.F.: 97231970589
Tel: +39 06 478361 - Fax: +39 06 47836320 -
Posta Elettronica Certificata:

CIVIC ACCESS PRESS REVIEW URP JOB OPPORTUNITIES CONTRACTS TRANSPARENT ADMINISTRATION

freccina You are here: Home->Publications->Datasheet


Publication datasheet
Title:
Biotechnological strategies and tools for Plum pox virus resistance: trans-, intra-, cis-genesis, and beyond
Authors:
Ilardi, V.; Tavazza, M.
Year:
2015
Languages:
ENG, eng
Journal:
Frontiers in Plant Science
Kind of publication:
Remoto
Location:
Editor:
Frontiers Research Foundation
Abstract in Italian:
Abstract in English:
Plum pox virus (PPV) is the etiological agent of sharka, the most devastating and economically important viral disease affecting Prunus species. It is widespread in most stone fruits producing countries even though eradication and quarantine programs are in place. The development of resistant cultivars and rootstocks remains the most ecologically and economically suitable approach to achieve long-term control of sharka disease. However, the few PPV resistance genetic resources found in Prunus germplasm along with some intrinsic biological features of stone fruit trees pose limits for efficient and fast breeding programs. This review focuses on an array of biotechnological strategies and tools, which have been used, or may be exploited to confer PPV resistance. A considerable number of scientific studies clearly indicate that robust and predictable resistance can be achieved by transforming plant species with constructs encoding intron-spliced hairpin RNAs homologous to conserved regions of the PPV genome. In addition, we discuss how recent advances in our understanding of PPV biology can be profitably exploited to develop viral interference strategies. In particular, genetic manipulation of host genes by which PPV accomplishes its infection cycle already permits the creation of intragenic resistant plants. Finally, we review the emerging genome editing technologies based on ZFN, TALEN and CRISPR/Cas9 engineered nucleases and how the knockout of host susceptibility genes will open up next generation of PPV resistant plants.
Link:
http://dx.doi.org/10.3389/fpls.2015.00379

AREA RISERVATA  Webmaster:
Logo mySQL Logo PHP