Microarray-based method for a single, multiple and simultaneous detection of fourteen artichoke viruses
Tiberini, A.; Barba, M.
Atti VIII International Symposium on Artichoke, Cardoon and their Wild Relatives
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Globe artichoke (Cynara scolymus L.) is a perennial vegetable crop which is affected by many viruses causing significant economic losses. Their detection and identification to reduce these losses is important to farmers, plant pathologists and others involved in plant protection activities of quarantine and certification programs.
The potentiality of an oligonucleotide diagnostic array based on the Combimatrix platform for the detection and identification of 14 artichoke virus species was examined.
More than 190 oligonucleotide virus probes (40-mer) were selected and used in the microarray diagnostic technique developed in this investigation. Most the probes were highly specific for detection, identification and discrimination the target viruses.
Further, for the first time the Combimatrix platform was applied directly to evaluate the sanitary status of artichoke samples collected during field surveys carried out in 5 different Italian Regions.
The repeatability, specificity and sensitivity of the oligonucleotide diagnostic array developed in this investigation demonstrated its applicability for routine diagnostic use in suspected artichoke germplasm as it detected simultaneously 14viruses in one single hybridization event.