Pyrenochaeta lycopersici genome assembly showed interesting feature related to its lifestyle and interaction with the host.
Aragona, M.; Minio, A.; Ferrarini, A.; Valente, M.T.; Bagnaresi, P.; Orrų, L.; Tononi, P.; Zamperin, G.; Infantino, A.; Valč, G.; Cattivelli, L.; Delledonne, M.
Journal of Plant Pathology
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Pyrenochaeta lycopersici is a soil-borne pathogen that causes
corky root rot disease in tomato (Solanum lycopersicum) and other
Solanaceous crops, reducing fruit yields by up to 75%. De novo
assembly of the P. lycopersici genome was based on Illumina sequencing
and the functional characterization of the draft sequence
by integrating RNA-Seq data was followed by an in-depth analysis
of the virulence mechanisms and potential pathogenicity effectors
encoded by this pathogen. We assembled a 54.9Mb P. lycopersici
draft genome and annotated approximately 17,000 genes. The P.
lycopersici genome is closely related to hemibiotrophs and necrotrophs,
in agreement with the phenotypic characteristics of the
fungus and its lifestyle. P. lycopersici genome reveals a significative
expansion of specific genes families related both to pathogenesis
and to reproduction mechanisms, including those responsible for
plant cell wall degradation, nutrient absorption and fungicide detoxification.
We also observed a significant expansion of the gene
families associated with heterokaryon incompatibility (HI), which
represents an important mechanism for this imperfect fungus for
increasing genetic variability. The assembly constitutes an important
resource to understand the molecular bases of corky root rot
and more in general to enrich current knowledge of plant-pathogen