A Survey for the main diseases of Limonium spp.
Rizzo, D.; Burchi, G.; Stefani, L.; Paoli, M.; Antonetti, M.; Teani, A.; Haegi, A.; Grassotti, A.
Journal of Plant Pathology
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Main fungal diseases that affect Limonium spp. are induced by Peronospora statices, Botrytis cinerea and Colletotrichum gloeosporioides. In the frame of a survey of a Limonium collection carried out by CRA-VIV at Pescia (PT), a screening was performed in May 2010 to verify the presence/susceptibility to the above-mentioned fungi. Seventyfour samples of symptomatic and asymptomatic material (leaves and stems) were collected from 20 different clones of Limonium, belonging to 5 different species and hybrids, including 6 commercial cultivars. DNA was extracted from plant material through a modified protocol based on CTAB 3%. Diagnosis was done with molecular biology protocols optimized in our laboratory for Colletotrichum gloeosporioides (PCR end point), Botrytis cinerea (PCR Real Time_SybrGreen) and with primers ITS4/6 that amplify oomycetes, to check for the presence of Peronospora statices. This latter assay was positive for 91.9% of samples, whereas Botrytis cinerea was detected in 60.8%, and Colletotrichum gloeosporioides in 38.2% of the samples. These results were confirmed by wet rooms tests. As to the visible effects of pathogenic fungi, none of the tested varieties of Limonium showed Peronospora statices symptoms; 12 clones were completely asymptomatic against Botrytis cinerea and Colletotrichum gloeosporioides (L. gmelinii, L. altaica L. serotinum and hybrids between them); 10 clones showed symptoms due to Botrytis cinerea (mostly L. latifolium, L. altaica, and only one clone of L. gmelinii); and 4 clones showed strong symptoms of both Botrytis cinerea and Colletotrichum gloeosporioides (L. latifolium and L. latifolium x otolepis).