Development of a oligonucleotide-based microarray for detection of multiple artichoke viruses
Tiberini, A.; Barba, M.
Journal of Plant Pathology
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Globe artichoke (Cynara scolymus) is a vegetable crop
affected by many viruses causing significant economic
losses. Virus detection and identification is important to
farmers, plant pathologists and others involved in plant
protection activities of quarantine and certification programs.
To this aim, the potentiality of an oligonucleotide
diagnostic array based on the Combimatrix platform for
the detection and identification of 14 artichoke-infecting
viruses was examined. More than 190 oligonucleotide
virus probes (40-mer) were selected and used in the microarray
diagnostic technique developed in this investigation.
Most of the probes were highly specific for detection,
identification and discrimination of target viruses.
Further, for the first time the Combimatrix platform
was applied directly to evaluate the sanitary status of artichoke
samples collected during field surveys carried
out in 6 different Italian regions. The repeatability, specificity
and sensitivity of the oligonucleotide diagnostic array
developed in this investigation demonstrated its applicability
for routine diagnostic use in artichoke
germplasm as it detected simultaneously 14 viruses in
one single hybridization event.