|Abstract in English:|
Euphresco is an EU-funded ERA-NET project with the aim of create a network of funders to coordinate the research on plant heath aspects. One of the research projects born in the mainframe of Euphresco is the project "Current and emerging Phytophthoras" (CEP), that involves different European countries and deals with Phytophthora species such as P. ramorum, P. kernoviae, and P. lateralis which are quarantine pathogens for UE member countries, and other current Phytophthoras. The topics are risk assessment, disease management, genotyping, but it focuses especially on the harmonization of diagnostic methods. Participants share procedures regarding both traditional methods, based on direct isolation and baiting methods, and molecular techniques. Within the latter, participants collaborate to improve techniques on DNA extraction, sample preparation and methodologies of detection, including field approaches such as LFD and LAMP. Our main work is addressed i) to improve the DNA extraction from soil by testing different methodologies, including commercial kits, on different soils, and ii) to set up a Macroarray detection platform which allows the simultaneous detection of several Phytophthora pathogens. This characteristic is very appreciable for complex diseases and/or for a faster and reliable diagnosis of several pathogens on the same matrix. We used the macroarry for the detection and identification of different Phytophthora species including P. ramorum, P. kernoviae, P. lateralis, but also other widespread species that are a serious threat in Italy such as P. cactorum and P. cinnamomi.