Occurence of Phomopsis sp. on Achillea Millefolium
Vrande?i?, K. ; Jurkovi?, D.; Riccioni, L.; ?osi?, J.; Pošti?, J.
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The Faculty of agriculture in Osijek and Agricultural Institute Osijek
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|Abstract in English:|
During a three year period (2004 to 2006) on locations of Eastern Croatia (Kneževi Vinogradi, Osijek, Bro?anci i Krndija) we collected plants of Achillea millefolium L. from which we isolated Phomopsis sp. Many of collected plants exhibited no visible symptoms of infection but in spring pycnidia were noticed on overwintered plants. Isolation was done on PDA from naturally infected plants and morphological and molecular characteristics were studied. Biometrical size of reproductive structures developed on naturally infected plants and grown on PDA were measured. Internal transcribed spacers ITS rDNA of examined isolates were sequenced. Although molecular analysis showed that isolates were grouped with Diaporthe arctii, we decided to classify isolates from A. millefolium as Phomopsis sp., the details of which were discussed in the paper.